diff --git a/doc/classes/TileSet.xml b/doc/classes/TileSet.xml
index a812b523078..a39a43be4c7 100644
--- a/doc/classes/TileSet.xml
+++ b/doc/classes/TileSet.xml
@@ -143,6 +143,14 @@
 				Returns the custom data layers count.
 			</description>
 		</method>
+		<method name="get_navigation_layer_layer_value" qualifiers="const">
+			<return type="bool" />
+			<param index="0" name="layer_index" type="int" />
+			<param index="1" name="layer_number" type="int" />
+			<description>
+				Returns whether or not the specified navigation layer of the TileSet navigation data layer identified by the given [param layer_index] is enabled, given a navigation_layers [param layer_number] between 1 and 32.
+			</description>
+		</method>
 		<method name="get_navigation_layer_layers" qualifiers="const">
 			<return type="int" />
 			<param index="0" name="layer_index" type="int" />
@@ -500,6 +508,15 @@
 				Sets the type of the custom data layer identified by the given index.
 			</description>
 		</method>
+		<method name="set_navigation_layer_layer_value">
+			<return type="void" />
+			<param index="0" name="layer_index" type="int" />
+			<param index="1" name="layer_number" type="int" />
+			<param index="2" name="value" type="bool" />
+			<description>
+				Based on [param value], enables or disables the specified navigation layer of the TileSet navigation data layer identified by the given [param layer_index], given a navigation_layers [param layer_number] between 1 and 32.
+			</description>
+		</method>
 		<method name="set_navigation_layer_layers">
 			<return type="void" />
 			<param index="0" name="layer_index" type="int" />
diff --git a/scene/resources/tile_set.cpp b/scene/resources/tile_set.cpp
index b5a68ef14bf..5f3a757c090 100644
--- a/scene/resources/tile_set.cpp
+++ b/scene/resources/tile_set.cpp
@@ -991,6 +991,28 @@ uint32_t TileSet::get_navigation_layer_layers(int p_layer_index) const {
 	return navigation_layers[p_layer_index].layers;
 }
 
+void TileSet::set_navigation_layer_layer_value(int p_layer_index, int p_layer_number, bool p_value) {
+	ERR_FAIL_COND_MSG(p_layer_number < 1, "Navigation layer number must be between 1 and 32 inclusive.");
+	ERR_FAIL_COND_MSG(p_layer_number > 32, "Navigation layer number must be between 1 and 32 inclusive.");
+
+	uint32_t _navigation_layers = get_navigation_layer_layers(p_layer_index);
+
+	if (p_value) {
+		_navigation_layers |= 1 << (p_layer_number - 1);
+	} else {
+		_navigation_layers &= ~(1 << (p_layer_number - 1));
+	}
+
+	set_navigation_layer_layers(p_layer_index, _navigation_layers);
+}
+
+bool TileSet::get_navigation_layer_layer_value(int p_layer_index, int p_layer_number) const {
+	ERR_FAIL_COND_V_MSG(p_layer_number < 1, false, "Navigation layer number must be between 1 and 32 inclusive.");
+	ERR_FAIL_COND_V_MSG(p_layer_number > 32, false, "Navigation layer number must be between 1 and 32 inclusive.");
+
+	return get_navigation_layer_layers(p_layer_index) & (1 << (p_layer_number - 1));
+}
+
 // Custom data.
 int TileSet::get_custom_data_layers_count() const {
 	return custom_data_layers.size();
@@ -3387,6 +3409,8 @@ void TileSet::_bind_methods() {
 	ClassDB::bind_method(D_METHOD("remove_navigation_layer", "layer_index"), &TileSet::remove_navigation_layer);
 	ClassDB::bind_method(D_METHOD("set_navigation_layer_layers", "layer_index", "layers"), &TileSet::set_navigation_layer_layers);
 	ClassDB::bind_method(D_METHOD("get_navigation_layer_layers", "layer_index"), &TileSet::get_navigation_layer_layers);
+	ClassDB::bind_method(D_METHOD("set_navigation_layer_layer_value", "layer_index", "layer_number", "value"), &TileSet::set_navigation_layer_layer_value);
+	ClassDB::bind_method(D_METHOD("get_navigation_layer_layer_value", "layer_index", "layer_number"), &TileSet::get_navigation_layer_layer_value);
 
 	// Custom data
 	ClassDB::bind_method(D_METHOD("get_custom_data_layers_count"), &TileSet::get_custom_data_layers_count);
diff --git a/scene/resources/tile_set.h b/scene/resources/tile_set.h
index c2ed798f2bd..2d8c2bd5bd7 100644
--- a/scene/resources/tile_set.h
+++ b/scene/resources/tile_set.h
@@ -475,6 +475,8 @@ public:
 	void remove_navigation_layer(int p_index);
 	void set_navigation_layer_layers(int p_layer_index, uint32_t p_layers);
 	uint32_t get_navigation_layer_layers(int p_layer_index) const;
+	void set_navigation_layer_layer_value(int p_layer_index, int p_layer_number, bool p_value);
+	bool get_navigation_layer_layer_value(int p_layer_index, int p_layer_number) const;
 
 	// Custom data
 	int get_custom_data_layers_count() const;