diff --git a/scene/resources/animation.cpp b/scene/resources/animation.cpp
index 7078d60de5e..b2051ded6e2 100644
--- a/scene/resources/animation.cpp
+++ b/scene/resources/animation.cpp
@@ -5554,10 +5554,10 @@ Variant Animation::subtract_variant(const Variant &a, const Variant &b) {
 			return (b.operator Quaternion()).inverse() * (a.operator Quaternion());
 		}
 		case Variant::TRANSFORM2D: {
-			return (b.operator Transform2D()).inverse() * (a.operator Transform2D());
+			return (b.operator Transform2D()).affine_inverse() * (a.operator Transform2D());
 		}
 		case Variant::TRANSFORM3D: {
-			return (b.operator Transform3D()).inverse() * (a.operator Transform3D());
+			return (b.operator Transform3D()).affine_inverse() * (a.operator Transform3D());
 		}
 		default: {
 			return Variant::evaluate(Variant::OP_SUBTRACT, a, b);