diff --git a/scene/2d/animated_sprite.cpp b/scene/2d/animated_sprite.cpp
index 4891823e318..5a618f8760e 100644
--- a/scene/2d/animated_sprite.cpp
+++ b/scene/2d/animated_sprite.cpp
@@ -599,6 +599,7 @@ void AnimatedSprite::play(const StringName &p_animation, const bool p_backwards)
 		}
 	}
 
+	is_over = false;
 	set_playing(true);
 }