diff --git a/tests/scene/test_image_texture_3d.h b/tests/scene/test_image_texture_3d.h
new file mode 100644
index 00000000000..f2a7abcf69f
--- /dev/null
+++ b/tests/scene/test_image_texture_3d.h
@@ -0,0 +1,101 @@
+/**************************************************************************/
+/*  test_image_texture_3d.h                                               */
+/**************************************************************************/
+/*                         This file is part of:                          */
+/*                             GODOT ENGINE                               */
+/*                        https://godotengine.org                         */
+/**************************************************************************/
+/* Copyright (c) 2014-present Godot Engine contributors (see AUTHORS.md). */
+/* Copyright (c) 2007-2014 Juan Linietsky, Ariel Manzur.                  */
+/*                                                                        */
+/* Permission is hereby granted, free of charge, to any person obtaining  */
+/* a copy of this software and associated documentation files (the        */
+/* "Software"), to deal in the Software without restriction, including    */
+/* without limitation the rights to use, copy, modify, merge, publish,    */
+/* distribute, sublicense, and/or sell copies of the Software, and to     */
+/* permit persons to whom the Software is furnished to do so, subject to  */
+/* the following conditions:                                              */
+/*                                                                        */
+/* The above copyright notice and this permission notice shall be         */
+/* included in all copies or substantial portions of the Software.        */
+/*                                                                        */
+/* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,        */
+/* EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF     */
+/* MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. */
+/* IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY   */
+/* CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT,   */
+/* TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE      */
+/* SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.                 */
+/**************************************************************************/
+
+#ifndef TEST_IMAGE_TEXTURE_3D_H
+#define TEST_IMAGE_TEXTURE_3D_H
+
+#include "core/io/image.h"
+#include "scene/resources/image_texture.h"
+
+#include "tests/test_macros.h"
+#include "tests/test_utils.h"
+
+namespace TestImageTexture3D {
+
+// [SceneTree] in a test case name enables initializing a mock render server,
+// which ImageTexture3D is dependent on.
+TEST_CASE("[SceneTree][ImageTexture3D] Constructor") {
+	Ref<ImageTexture3D> image_texture_3d = memnew(ImageTexture3D);
+	CHECK(image_texture_3d->get_format() == Image::FORMAT_L8);
+	CHECK(image_texture_3d->get_width() == 1);
+	CHECK(image_texture_3d->get_height() == 1);
+	CHECK(image_texture_3d->get_depth() == 1);
+	CHECK(image_texture_3d->has_mipmaps() == false);
+}
+
+TEST_CASE("[SceneTree][ImageTexture3D] get_format") {
+	Ref<ImageTexture3D> image_texture_3d = memnew(ImageTexture3D);
+	CHECK(image_texture_3d->get_format() == Image::FORMAT_L8);
+}
+
+TEST_CASE("[SceneTree][ImageTexture3D] get_width") {
+	Ref<ImageTexture3D> image_texture_3d = memnew(ImageTexture3D);
+	CHECK(image_texture_3d->get_width() == 1);
+}
+
+TEST_CASE("[SceneTree][ImageTexture3D] get_height") {
+	Ref<ImageTexture3D> image_texture_3d = memnew(ImageTexture3D);
+	CHECK(image_texture_3d->get_height() == 1);
+}
+
+TEST_CASE("[SceneTree][ImageTexture3D] get_depth") {
+	Ref<ImageTexture3D> image_texture_3d = memnew(ImageTexture3D);
+	CHECK(image_texture_3d->get_depth() == 1);
+}
+
+TEST_CASE("[SceneTree][ImageTexture3D] has_mipmaps") {
+	const Vector<Ref<Image>> images = { memnew(Image(8, 8, false, Image::FORMAT_RGBA8)), memnew(Image(8, 8, false, Image::FORMAT_RGBA8)) };
+	Ref<ImageTexture3D> image_texture_3d = memnew(ImageTexture3D);
+	CHECK(image_texture_3d->has_mipmaps() == false); // No mipmaps.
+	image_texture_3d->create(Image::FORMAT_RGBA8, 2, 2, 2, true, images);
+	CHECK(image_texture_3d->has_mipmaps() == true); // Mipmaps.
+}
+
+TEST_CASE("[SceneTree][ImageTexture3D] create") {
+	const Vector<Ref<Image>> images = { memnew(Image(8, 8, false, Image::FORMAT_RGBA8)), memnew(Image(8, 8, false, Image::FORMAT_RGBA8)) };
+	Ref<ImageTexture3D> image_texture_3d = memnew(ImageTexture3D);
+	CHECK(image_texture_3d->create(Image::FORMAT_RGBA8, 2, 2, 2, true, images) == OK); // Run create and check return value simultaneously.
+	CHECK(image_texture_3d->get_format() == Image::FORMAT_RGBA8);
+	CHECK(image_texture_3d->get_width() == 2);
+	CHECK(image_texture_3d->get_height() == 2);
+	CHECK(image_texture_3d->get_depth() == 2);
+	CHECK(image_texture_3d->has_mipmaps() == true);
+}
+
+TEST_CASE("[SceneTree][ImageTexture3D] set_path") {
+	Ref<ImageTexture3D> image_texture_3d = memnew(ImageTexture3D);
+	String path = TestUtils::get_data_path("images/icon.png");
+	image_texture_3d->set_path(path, true);
+	CHECK(image_texture_3d->get_path() == path);
+}
+
+} //namespace TestImageTexture3D
+
+#endif // TEST_IMAGE_TEXTURE_3D_H
diff --git a/tests/test_main.cpp b/tests/test_main.cpp
index 041231888b2..3c875797a49 100644
--- a/tests/test_main.cpp
+++ b/tests/test_main.cpp
@@ -111,6 +111,7 @@
 #include "tests/scene/test_curve_3d.h"
 #include "tests/scene/test_gradient.h"
 #include "tests/scene/test_image_texture.h"
+#include "tests/scene/test_image_texture_3d.h"
 #include "tests/scene/test_instance_placeholder.h"
 #include "tests/scene/test_node.h"
 #include "tests/scene/test_node_2d.h"